Recombinant humaan CTH-eiwit

CTH (Cystathionine gamma-lyase or cystathionase) belongs to the trans-sulfuration enzymes family. This protein is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. CTH catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Recombinant human CTH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cystathionine gamma-lyase, Cystathionine gamma-lyase, Gamma-cystathionase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

46.7 kDa (425aa) confirmed by MALDI-TOF

CTH, Cystathionine gamma-lyase, Cystathionine gamma-lyase, Gamma-cystathionase, ATGP1333-10 µg, ATGP1333-20 µg, ATGP1333-50 µg, ATGP1333-100 µg, ATGP1333-250 µg, ATGP1333-500 µg, ATGP1333-1 mg, ATGP1333-10, ATGP1333-20, ATGP1333-50, ATGP1333-100, ATGP1333-250, ATGP1333-500, ATGP1333-1

Chiku T., et al. (2009) J. Biol. Chem. 284:11601-11612; ; Sun Q., et al. (2009) J. Biol. Chem. 284:3076-3085

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_001893.2

P32929

Human

MQEKDASSQG FLPHFQHFAT QAIHVGQDPE QWTSRAVVPP ISLSTTFKQG APGQHSGFEY SRSGNPTRNC LEKAVAALDG AKYCLAFASG LAATVTITHL LKAGDQIICM DDVYGGTNRY FRQVASEFGL KISFVDCSKI KLLEAAITPE TKLVWIETPT NPTQKVIDIE GCAHIVHKHG DIILVVDNTF MSPYFQRPLA LGADISMYSA TKYMNGHSDV VMGLVSVNCE SLHNRLRFLQ NSLGAVPSPI DCYLCNRGLK TLHVRMEKHF KNGMAVAQFL ESNPWVEKVI YPGLPSHPQH ELVKRQCTGC TGMVTFYIKG TLQHAEIFLK NLKLFTLAES LGGFESLAEL PAIMTHASVL KNDRDVLGIS DTLIRLSVGL EDEEDLLEDL DQALKAAHPP SGSHS