Recombinant humaan CTLA-4 eiwit

CTLA-4, also known as cytotoxic T-lymphocyte protein 4, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells. It also is a protein receptor that functions as an immune checkpoint and downregulates immune responses. This protein is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation a phenomenon which is particularly notable in cancers. It may also function via modulation of cell motility and signaling through PI3 kinase. It has been implicated in several autoimmune diseases, such as systemic lupus erythematosus. Recombinant human CTLA-4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CTLA-4 inhibits Human B7-1/CD80 induced IL-2 secretion of the Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/mL.

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

The activity is determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/mL with Human B7-1/CD80.

14.6 kDa (135aa)

Cytotoxic T lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA 4, CTLA4, CTLA-4, CD 152, CD152, CELIAC3, CELIAC 3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, Insulin dependent diabetes mellitus 12, IDDM 12, IDDM12, CD, GSE, ATGP3918-10 µg, ATGP3918-20 µg, ATGP3918-50 µg, ATGP3918-100 µg, ATGP3918-250 µg, ATGP3918-500 µg, ATGP3918-1 mg, ATGP3918-010, ATGP3918-020, ATGP3918-050, ATGP3918-100, ATGP3918-250, ATGP3918-500, ATGP3918-01M, ATGP3334

Dai F., et al. (2015) Braz J Med Biol Res. 48:629-636. ; ; Ward FJ., et al. (2013) Eur J Immunol. 43:1274-1285.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Immunology

NP_005205.2

P16410

Human

Ala

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