Recombinant humaan CTLA4-eiwit

CTLA4 is a member of the immunoglobulin superfamily and transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. Recombinant human CTLA4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.9 kDa (149aa)

CTLA4, Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, CD, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12, ATGP1959-10 µg, ATGP1959-20 µg, ATGP1959-50 µg, ATGP1959-100 µg, ATGP1959-250 µg, ATGP1959-500 µg, ATGP1959-1 mg, ATGP1959-10, ATGP1959-20, ATGP1959-50, ATGP1959-100, ATGP1959-250, ATGP1959-500, ATGP1959-1

Rudd CE, Taylor A, et al. (2009) . Immunol Rev. 229 (1) :12-26.; ; Stamper CC, Zhang Y, et al. (2001) . Nature. 410 (6828) :608-11.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Immunology

NP_005205.2

P16410

Human

KAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSD