Recombinant humaan CTLA4-eiwit

CTLA-4, also known as cytotoxic T-lymphocyte protein 4, is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. The affinity of CTLA-4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Recombinant human CTLA-4, fused to hIgG His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CTLA-4 inhibits Human B7 1/CD80 induced IL-2 secretion of the Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/mL.

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

The activity is determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/mL with Human B7 1/CD80.

40.8 kDa (368aa)

Cytotoxic T lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA 4, CTLA4, CTLA-4, CD 152, CD152, CELIAC3, CELIAC 3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, Insulin dependent diabetes mellitus 12, IDDM 12, IDDM12, CD, GSE, ATGP3914-10 µg, ATGP3914-20 µg, ATGP3914-50 µg, ATGP3914-100 µg, ATGP3914-250 µg, ATGP3914-500 µg, ATGP3914-1 mg, ATGP3914-010, ATGP3914-020, ATGP3914-050, ATGP3914-100, ATGP3914-250, ATGP3914-500, ATGP3914-01M, ATGP3364

Harper K., et al. (1991) J. Immunol. 147:1037-1044.; ; Teft WA., et al. (2006) Annu. Rev. Immunol. 24:65-97.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_005205

P16410

Human

Ala

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