Recombinant humaan CTRP5/C1qTNF5 eiwit

CTRP5, also known as Complement C1q tumor necrosis factor-related protein 5, encodes a short-chain collagen which is strongly expressed in sub-retinal pigment epithelium (sub-RPE), ciliary epithelium and adipose tissue. CTRP5 is increased in mtDNA-depleted myocytes and that it stimulates the phosphorylation of AMP activated protein kinase. CTRP5 plays an important role in the adhesion of the retinal pigment epithelium (RPE) to the Bruch Membrane, and mutations are thought to impair the adhesion, resulting in sub-RPE deposits. Recombinant human CTRP5, fused to His-tag at N-terminals, was expressed in E. coli and purified by conventional chromatography techniques.

Complement C1q tumor necrosis factor-related protein 5, CTRP5, MFRP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

26.4 kDa (253aa) confirmed by MALDI-TOF

CTRP5, C1qTNF5, CTRP5/C1qTNF5Complement C1q tumor necrosis factor-related protein 5, CTRP5, MFRP, ATGP3859-10 µg, ATGP3859-20 µg, ATGP3859-50 µg, ATGP3859-100 µg, ATGP3859-250 µg, ATGP3859-500 µg, ATGP3859-1 mg, ATGP3859-10, ATGP3859-20, ATGP3859-50, ATGP3859-100, ATGP3859-250, ATGP3859-500, ATGP3859-1

Park SY., et al. (2009) J Biol Chem. 284 (41) :27780-9.; ; Hayward C., et al. (2003) Hum Mol Genet. 12 (20) :2657-67.; ; Ayyagari R., et al. (2005) Invest Ophthalmol Vis Sci. 46 (9) :3363-71.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 30% glycerol, 0.2M NaCl

Immunology

NP_001265360

Q9BXJ0

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSPPLD DNKIPSLCPG HPGLPGTPGH HGSQGLPGRD GRDGRDGAPG APGEKGEGGR PGLPGPRGDP GPRGEAGPAG PTGPAGECSV PPRSAFSAKR SESRVPPPSD APLPFDRVLV NEQGHYDAVT GKFTCQVPGV YYFAVHATVY RASLQFDLVK NGESIASFFQ FFGGWPKPAS LSGGAMVRLE PEDQVWVQVG VGDYIGIYAS IKTDSTFSGF LVYSDWHSSP VFA