Recombinant humaan CUEDC1-eiwit

CuEDC1 contains 1 CuE domain. The CuE domain is structurally related to the ubiquitin-binding uBA domain and has been reported to exist as a domain-swapped dimer that makes additional contacts with ubiquitin, and consequently, binds ubiquitin with higher affinity. The CuE domain is found in proteins with diverse functions including degradation of misfolded proteins in the endoplasmic reticulum and protein sorting. Recombinant human CuEDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CUE domain-containing protein 1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

44.6 kDa (409aa) confirmed by MALDI-TOF

CuEDC1, CUE domain-containing protein 1, ATGP2492-10 µg, ATGP2492-20 µg, ATGP2492-50 µg, ATGP2492-100 µg, ATGP2492-250 µg, ATGP2492-500 µg, ATGP2492-1 mg, ATGP2492-010, ATGP2492-020, ATGP2492-050, ATGP2492-100, ATGP2492-250, ATGP2492-500, ATGP2492-01M

Gerhard DS, Wagner L, et al. (2004) . Genome Res. 14 (10B) :2121-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Other

NP_001258804

Q9NWM3

Human

MGSSHHHHHH SSGLVPRGSH MGSMTSLFRR SSSGSGGGGT AGARGGGGGT AAPQELNNSR PARQVRRLEF NQAMDDFKTM FPNMDYDIIE CVLRANSGAV DATIDQLLQM NLEGGGSSGG VYEDSSDSED SIPPEILERT LEPDSSDEEP PPVYSPPAYH MHVFDRPYPL APPTPPPRID ALGSGAPTSQ RRYRNWNPPL LGNLPDDFLR ILPQQLDSIQ GNAGGPKPGS GEGCPPAMAG PGPGDQESRW KQYLEDERIA LFLQNEEFMK ELQRNRDFLL ALERDRLKYE SQKSKSSSVA VGNDFGFSSP VPGTGDANPA VSEDALFRDK LKHMGKSTRR KLFELARAFS EKTKMRKSKR KHLLKHQSLG AAASTANLLD DVEGHACDED FRGRRQEAPK VEEGLREGQ