Recombinant humaan CUEDC2-eiwit

CUEDC2 belongs to the CUEDC2 family and contains 1 CUE domain. This protein down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. It also involves in 17 beta-estradiol-induced ESR1 degradation and controls PGR protein levels through a similar mechanism. Recombinant human CUEDC2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CuE domain-containing protein 2, bA18I14.5, C10orf66

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.4 kDa (310aa) confirmed by MALDI-TOF

CUEDC2, CuE domain-containing protein 2, bA18I14.5, C10orf66, ATGP2299-10 µg, ATGP2299-20 µg, ATGP2299-50 µg, ATGP2299-100 µg, ATGP2299-250 µg, ATGP2299-500 µg, ATGP2299-1 mg, ATGP2299-10, ATGP2299-20, ATGP2299-50, ATGP2299-100, ATGP2299-250, ATGP2299-500, ATGP2299-1

Pan X, Zhou T, et al. (2011) . Nat Med. 17 (6) :708-14. ; ; Zhang PJ, Zhao J, et al. (2007) . EMBO J. 26 (7) :1831-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_076945

Q9H467

Human

MELERIV SAALLAFVQT HLPEADLSGL DEVIFSYVLG VLEDLGPSGP SEENFDMEAF TEMMEAYVPG FAHIPRGTIG DMMQKLSGQL SDARNKENLQ PQSSGVQGQV PISPEPLQRP EMLKEETRSS AAAAADTQDE ATGAEEELLP GVDVLLEVFP TCSVEQAQWV LAKARGDLEE AVQMLVEGKE EGPAAWEGPN QDLPRRLRGP QKDELKSFIL QKYMMVDSAE DQKIHRPMAP KEAPKKLIRY IDNQVVSTKG ERFKDVRNPE AEEMKATYIN LKPARKYRFH