Recombinant humaan CuL1-eiwit

Cullin1, also known CuL1, is a core component of multiple cullin-RING-based SCF (SKP1-CuL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, it serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cullin1 may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Recombinant human CuL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cullin-1, CuL-1, Cullin-1, MGC149834, MGC149835

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

49.4 kDa (430aa) confirmed by MALDI-TOF

CuL1, Cullin-1, CuL-1, Cullin-1, MGC149834, MGC149835, ATGP0753-10 µg, ATGP0753-20 µg, ATGP0753-50 µg, ATGP0753-100 µg, ATGP0753-250 µg, ATGP0753-500 µg, ATGP0753-1 mg, ATGP0753-10, ATGP0753-20, ATGP0753-50, ATGP0753-100, ATGP0753-250, ATGP0753-500, ATGP0753-1

Michel JJ., et al. (1998) Cell Growth Differ. 9 (6) :435-49.; ; Kipreos ET., et al. (1996) Cell. 85 (6) :829-39.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Cell Cycle

NP_003583

Q13616

Human

MSSTRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY NYCTSVHQSN QARGAGVPPS KSKKGQTPGG AQFVGLELYK RLKEFLKNYL TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD EGRKGIYEIY SLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL ISGVVQSYVE LGLNEDDAFA KGPTLTVYKE SFESQFLADT ERFYTRESTE FLQQNPVTEY MKKAEARLLE EQRRVQVYLH ESTQDELARK CEQVLIEKHL EIFHTEFQNL LDADKNEDLG RMYNLVSRIQ DGLGELKKLL ETHIHNQGLA AIEKCGEAAL NDPKMYVQTV LDVHKKYNAL VMSAFNNDAG FVAALDKACG RFINNNAVTK