Recombinant humaan CUTC-eiwit

CuTC is a member of the Cut family and is suggested to be involved in efflux trafficking of cuprous ion. The Cut family was associated with the copper homeostasis and involved in several important metabolisms, such as uptake, storage, delivery, and efflux of copper. Copper is an essential heavy metal trace element that plays important roles in cell physiology. Recombinant human CuTC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Copper homeostasis protein cutC homolog, CGI-32

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.5 kDa (293a) confirmed by MALDI-TOF

CUTC, copper homeostasis protein cutC homolog, CGI-32, ATGP1181-10 µg, ATGP1181-20 µg, ATGP1181-50 µg, ATGP1181-100 µg, ATGP1181-250 µg, ATGP1181-500 µg, ATGP1181-1 mg, ATGP1181-10, ATGP1181-20, ATGP1181-50, ATGP1181-100, ATGP1181-250, ATGP1181-500, ATGP1181-1

Li J., et al. (2005) Biochem. Biophys. Res. Commun. 337:179-183; ; Li Y., et al. (2010) J. Struct. Biol. 169:399-405

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200 mM NaCl

Metabolism

NP_057044

Q9NTM9

Human

MGSSHHHHHH SSGLVPRGSH MKRQGASSER KRARIPSGKA GAANGFLMEV CVDSVESAVN AERGGADRIE LCSGLSEGGT TPSMGVLQVV KQSVQIPVFV MIRPRGGDFL YSDREIEVMK ADIRLAKLYG ADGLVFGALT EDGHIDKELC MSLMAICRPL PVTFHRAFDM VHDPMAALET LLTLGFERVL TSGCDSSALE GLPLIKRLIE QAKGRIVVMP GGGITDRNLQ RILEGSGATE FHCSARSTRD SGMKFRNSSV AMGASLSCSE YSLKVTDVTK VRTLNAIAKN ILV