Recombinant humaan CX3CL1/fractalkine-eiwit

CX3CL1, also known as fractalkine. CX3CL1 has a unique CX3C cysteine motif near the amino terminus and is the first member of a fourth branch of the chemokine superfamily. unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin like stalk. It is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Also, CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Recombinant human CX3CL1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Chemokine (C-X3-C motif) ligand 1, ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, CX3CL1, Chemokine (C-X3-C motif) ligand 1, small inducible cytokine subfamily D (Cys-X3-Cys) member 1 (fractalkine, neurotactin), fractalkine, neurotactin

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

10.9 kDa (97aa) confirmed by MALDI-TOF

CX3CL1, Fractalkine, Chemokine (C-X3-C motif) ligand 1, ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, CX3CL1, Chemokine (C-X3-C motif) ligand 1, small inducible cytokine subfamily D (Cys-X3-Cys) member 1 (fractalkine, neurotactin), fractalkine, neurotactin, ATGP0380-10 µg, ATGP0380-20 µg, ATGP0380-50 µg, ATGP0380-100 µg, ATGP0380-250 µg, ATGP0380-500 µg, ATGP0380-1 mg, ATGP0380-10, ATGP0380-20, ATGP0380-50, ATGP0380-100, ATGP0380-250, ATGP0380-500, ATGP0380-1

Bazan JF., et al. (1997) Nature. 385 (6617) :640-4. ; ; Papadopoulos EJ., et al. (1999) Eur J Immunol. 29 (8) :2551-9. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Hormones & Cytokines

NP_002987

P78423

Human

MGSSHHHHHH SSGLVPRGSH MQHHGVTKCN ITCSKMTSKI PVALLIHYQQ NQASCGKRAI ILETRQHRLF CADPKEQWVK DAMQHLDRQA AALTRNG