Recombinant humaan CX3CL1/fractalkine-eiwit

CX3CL1, also known as fractalkine isoform 1, is a member of the CX3C chemokine family. It acts as a ligand for both CX3CR1 and integrins. This protein acts different ways dependent on its form. The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. This protein plays a role in regulating leukocyte adhesion and migration processes at the endothelium. It is also up-regulated in the hippocampus during a brief temporal window following spatial learning, the purpose of which may be to regulate glutamate-mediated neurotransmission tone. This indicates a possible role for the chemokine in the protective plasticity process of synaptic scaling. Recombinant human CX3CL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography technique.

CX3CL1, ABCD-3, C3Xkine, CXC3, CXC3C, fractalkine, neurotactin, NTN, NTT, SCYD1, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

34.3 kDa (323aa)

CX3CL1, Fractalkine, ATGP3885-10 µg, ATGP3885-20 µg, ATGP3885-50 µg, ATGP3885-100 µg, ATGP3885-250 µg, ATGP3885-500 µg, ATGP3885-1 mg, ATGP3885-10, ATGP3885-20, ATGP3885-50, ATGP3885-100, ATGP3885-250, ATGP3885-500, ATGP3885-1, ATGP3885-10 µg, ATGP3885-20 µg, ATGP3885-50 µg, ATGP3885-100 µg, ATGP3885-250 µg, ATGP3885-500 µg, ATGP3885-1 mg, ATGP3885-10, ATGP3885-20, ATGP3885-50, ATGP3885-100, ATGP3885-250, ATGP3885-500, ATGP3885-1

Conroy MJ., et al. (2018) Front Immunol. 9:1867. ; ; Hoffmann-Vold AM., et al. (2018) PLoS One. 13:e0206545.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_002987

P78423

Human

Gln25