Recombinant humaan CYB5R1-eiwit

Cytochrome b5 reductase 1, also known as CYB5R1, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. This protein is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Recombinant human CYB5R1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytochrome b5 reductase 1, B5R.1, B5R1, B5R2, humb5R2, NQO3A2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

33.8 kDa (300aa) confirmed by MALDI-TOF

CYB5R1, Cytochrome b5 reductase 1, B5R.1, B5R1, B5R2, humb5R2, NQO3A2, ATGP2066-10 µg, ATGP2066-20 µg, ATGP2066-50 µg, ATGP2066-100 µg, ATGP2066-250 µg, ATGP2066-500 µg, ATGP2066-1 mg, ATGP2066-10, ATGP2066-20, ATGP2066-50, ATGP2066-100, ATGP2066-250, ATGP2066-500, ATGP2066-1

Zhu H., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:14742-14747; ; Clark H.F., et al. (2003) Genome Res. 13:2265-2270;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_057327

Q9UHQ9

Human

MGSSHHHHHH SSGLVPRGSH MGSLVRRSRR PQVTLLDPNE KYLLRLLDKT TVSHNTKRFR FALPTAHHTL GLPVGKHIYL STRIDGSLVI RPYTPVTSDE DQGYVDLVIK VYLKGVHPKF PEGGKMSQYL DSLKVGDVVE FRGPSGLLTY TGKGHFNIQP NKKSPPEPRV AKKLGMIAGG TGITPMLQLI RAILKVPEDP TQCFLLFANQ TEKDIILRED LEELQARYPN RFKLWFTLDH PPKDWAYSKG FVTADMIREH LPAPGDDVLV LLCGPPPMVQ LACHPNLDKL GYSQKMRFTY