Recombinant humaan CYB5R2-eiwit

Cytochrome b5 reductase 2, also known as CYB5R2, is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. This protein is responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2, 4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) . Recombinant human CYB5R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytochrome b5 reductase 2, B5R.2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

33.8 kDa (299aa) confirmed by MALDI-TOF

CYB5R2, Cytochrome b5 reductase 2, B5R.2, ATGP2679-10 µg, ATGP2679-20 µg, ATGP2679-50 µg, ATGP2679-100 µg, ATGP2679-250 µg, ATGP2679-500 µg, ATGP2679-1 mg, ATGP2679-10, ATGP2679-20, ATGP2679-50, ATGP2679-100, ATGP2679-250, ATGP2679-500, ATGP2679-1

Baker M.A., et al. (2005) Biol. Reprod. 73:334-342; ; Zhu H., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:14742-14747;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cardiovascular

NP_057313

Q6BCY4

Human

MNSRRRE PITLQDPEAK YPLPLIEKEK ISHNTRRFRF GLPSPDHVLG LPVGNYVQLL AKIDNELVVR AYTPVSSDDD RGFVDLIIKI YFKNVHPQYP EGGKMTQYLE NMKIGETIFF RGPRGRLFYH GPGNLGIRPD QTSEPKKTLA DHLGMIAGGT GITPMLQLIR HITKDPSDRT RMSLIFANQT EEDILVRKEL EEIARTHPDQ FNLWYTLDRP PIGWKYSSGF VTADMIKEHL PPPAKSTLIL VCGPPPLIQT AAHPNLEKLG YTQDMIFTY