Recombinant humaan CYB5R3-eiwit

CYB5R3 is a cytochrome b5 reductasecytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Mutations in this gene cause methemoglobinemias. Recombinant human CYB5R3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytochrome b5 reductase 3., B5R, DIA1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

34 kDa (300aa) confirmed by MALDI-TOF

CYB5R3, Cytochrome b5 reductase 3., B5R, DIA1, ATGP1380-10 µg, ATGP1380-20 µg, ATGP1380-50 µg, ATGP1380-100 µg, ATGP1380-250 µg, ATGP1380-500 µg, ATGP1380-1 mg, ATGP1380-10, ATGP1380-20, ATGP1380-50, ATGP1380-100, ATGP1380-250, ATGP1380-500, ATGP1380-1

Percy MJ., et al. (2006) Arch Biochem Biophys. 447 (1) :59-67.; ; Roma GW., et al. (2006) Arch Biochem Biophys. 452 (1) :69-82.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Metabolism

NP_000389.1

P00387

Human

FQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RFKLWYTLDR APEAWDYGQG FVNEEMIRDH LPPPEEEPLV LMCGPPPMIQ YACLPNLDHV GHPTERCFVF