Recombinant humaan Cycline H-eiwit

Cyclin H, also known as CDK7, is a member of the cyclin-dependent protein kinase family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin H forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK) . Cyclin H is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. Recombinant human Cyclin H, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

39.8 kDa (343aa) confirmed by MALDI-TOF

Cyclin H, CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H, ATGP0525-10 µg, ATGP0525-20 µg, ATGP0525-50 µg, ATGP0525-100 µg, ATGP0525-250 µg, ATGP0525-500 µg, ATGP0525-1 mg, ATGP0525-10, ATGP0525-20, ATGP0525-50, ATGP0525-100, ATGP0525-250, ATGP0525-500, ATGP0525-1

Schneider, E., et al. (1998) Oncogene. 17 (21) :2733-41.; ; Yee A., et al. (1995) Canser Res. 55 (24) :6058-62.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 2mM EDTA, 0.1M NaCl

Cell Cycle

NP_001230

P51946

Human

MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD DYVSKKSKHE EEEWTDDDLV ESL