Recombinant humaan Cyclofiline-achtig 2/PPIL2-eiwit

PPIL2, which belongs to the cyclophilin family of peptidylprolyl isomerases, is an enzyme that plays an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Recombinant PPIL2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 290 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin..

UBOX7, U-box domain containing 7, RING-type E3 ubiquitin-protein ligase PPIL2, PPIase, Peptidylprolyl isomerase like 2, Peptidyl-prolyl cis-trans isomerase-like 2, hCyP-60, Cyp60, Cyclophilin-like protein Cyp-60, Cyclophilin-60, CYC4

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 290 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin..

61.6 kDa (547aa)

PPIL2, Peptidyl-prolyl cis-trans isomerase-like 2, CYC4, Cyp-60, CYP60, hCyP-60, GC33174, Peptidyl-prolyl cis-trans isomerase-like 2 Cyclophilin 60, Cyclophilin like protein Cyp 60, CYP60, Peptidyl prolyl cis trans isomerase like 2, PPIase, Rotamase PPIL2., ATGP0498-10 µg, ATGP0498-20 µg, ATGP0498-50 µg, ATGP0498-100 µg, ATGP0498-250 µg, ATGP0498-500 µg, ATGP0498-1 mg, ATGP0498-10, ATGP0498-20, ATGP0498-50, ATGP0498-100, ATGP0498-250, ATGP0498-500, ATGP0498-1

Wang BB, et al. (1996) Biochem J. 314:313-9.; ; Carson R, et al. (2009) Neuromolecular Med. 11 (4) :337-44.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Heat Shock Proteins

NP_680481.1

Q13356

Human

MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH