Recombinant humaan Cyclofiline-achtig 3/PPIL3-eiwit

PPIL3, also known as peptidyl-prolyl cis-trans isomerase-like 3, is a member of the cyclophilin family that catalyzes the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. It has been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Recombinant human PPIL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 280 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

Peptidyl-prolyl cis-trans isomerase-like 3 isoform PPIL3b, CYPJ, Peptidyl-prolyl cis-trans isomerase-like 3, PPIase, Cyclophilin J, Cyclophilin-like protein PPIL3, Rotamase PPIL3

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 280 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

20.3 kDa (181aa) confirmed by MALDI-TOF

PPIL3, Peptidyl-prolyl cis-trans isomerase-like 3 isoform PPIL3b, CYPJ, ATGP1230-10 µg, ATGP1230-20 µg, ATGP1230-50 µg, ATGP1230-100 µg, ATGP1230-250 µg, ATGP1230-500 µg, ATGP1230-1 mg, ATGP1230-10, ATGP1230-20, ATGP1230-50, ATGP1230-100, ATGP1230-250, ATGP1230-500, ATGP1230-1

Zhou Z. et al. (2001) Cytogenet Cell Genet. 92: 231-236; ; Jurica MS. et al. (2002) RNA. 8:426-439.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Heat Shock Proteins

NP_570981

Q9H2H8

Human

MSVTLHTDVG DIKIEVFCER TPKTCENFLA LCASNYYNGC IFHRNIKGFM VQTGDPTGTG RGGNSIWGKK FEDEYSEYLK HNVRGVVSMA NNGPNTNGSQ FFITYGKQPH LDMKYTVFGK VIDGLETLDE LEKLPVNEKT YRPLNDVHIK DITIHANPFA Q