Recombinant humaan Cyclofiline-achtig 4/PPIL4-eiwit

PPIL4, also known as HDCME13P, is evolutionarily conserved member of the cyclophilin-type PPIase family of proteins. ubiquitously expressed with predominant expression in kidney, PPIL4 localizes to the nucleus and contains one PPIase cyclophilin-type domain, a lysine-rich domain, a pair of bipartite nuclear targeting sequences and one RRM (RNA recognition motif) domain. The presence of the RRM domain along with nuclear targeting sequences suggests that PPIL4 may be involved in transcriptional regulation. Recombinant human PPIL4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 190 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin

Peptidyl-prolyl cis-trans isomerase-like 4, HDCME13P, Rotamase PPIL4, Cyclophilin-like protein PPIL4, PPIase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

Specific activity is > 190 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin

59.3 kDa (512aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PPIL4, Peptidyl-prolyl cis-trans isomerase-like 4, HDCME13P, Rotamase PPIL4, Cyclophilin-like protein PPIL4, ATGP0791-10 µg, ATGP0791-20 µg, ATGP0791-50 µg, ATGP0791-100 µg, ATGP0791-250 µg, ATGP0791-500 µg, ATGP0791-1 mg, ATGP0791-10, ATGP0791-20, ATGP0791-50, ATGP0791-100, ATGP0791-250, ATGP0791-500, ATGP0791-1

Nebert DW., et al. (2004) Hum Genomics. 1 (5) :381-8. ; ; Valle C., et al. (2005) Parasitol Res. 96 (4) :199-205.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Heat Shock Proteins

NP_624311.1

Q8WUA2

Human

MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR