Recombinant humaan Cyclofiline G/PPIG-eiwit

Peptidyl-prolyl cis-trans isomerase G (PPIG), also known as Cyclophilin G, is a member of peptidyl-prolyl cis-trans isomerase family (PPIases) . This protein catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is localized to the nuclear speckles, a nuclear compartment rich in splicing factors, and interacts with the splicing factors SC35 and pinin. Cyclophilin G also may play an important role in the regulation of pre-mRNA splicing. Recombinant human Cyclophilin G, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 600 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Peptidyl-prolyl cis-trans isomerase G, PPIase G, Rotamase G, Cyclophilin G, CASP10, PPIG, Peptidyl-prolyl cis-trans isomerase G, peptidylprolyl isomerase G (cyclophilin G), CARS-Cyp, SRCyp, SCAF10, SR-related CTD-associated factor 10

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 600 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

21.6 kDa (195aa) confirmed by MALDI-TOF

Cyclophilin G, PPIG, Peptidyl-prolyl cis-trans isomerase G, PPIase G, Rotamase G, Cyclophilin G, CASP10, PPIG, Peptidyl-prolyl cis-trans isomerase G, peptidylprolyl isomerase G (cyclophilin G), CARS-Cyp, SRCyp, SCAF10, SR-related CTD-associated factor 10, ATGP0431-10 µg, ATGP0431-20 µg, ATGP0431-50 µg, ATGP0431-100 µg, ATGP0431-250 µg, ATGP0431-500 µg, ATGP0431-1 mg, ATGP0431-10, ATGP0431-20, ATGP0431-50, ATGP0431-100, ATGP0431-250, ATGP0431-500, ATGP0431-1

Modjtahedi N., et al. (2004) . J Biol Chem. 279 (21) :22322-30 ; ; Ouyang P., et al. (2004) . Biochem Biophys Re Commun. 321 (3) : 638-47 ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Heat Shock Proteins

AAH01555

Q13427

Human

MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG