Recombinant humaan Cystatine B-eiwit

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus. Recombinant CSTB, fused to His-tag, was expressed in E. coli and purified by using conventional chromatography techniques.

The IC50 value is < 3.0 nm. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Cystatin-B, Stefin B, PME, CST6

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

The IC50 value is < 3.0 nm. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

13 kDa (118aa) confirmed by MALDI-TOF

Cystatin B, Cystatin-B, Stefin B, PME, CST6, ATGP3262-10 µg, ATGP3262-20 µg, ATGP3262-50 µg, ATGP3262-100 µg, ATGP3262-250 µg, ATGP3262-500 µg, ATGP3262-1 mg, ATGP3262-10, ATGP3262-20, ATGP3262-50, ATGP3262-100, ATGP3262-250, ATGP3262-500, ATGP3262-1

Turk V, et al (1991) FEBS Lett. 285 (2) : 213-9. ; ; Jarvinen M, et al, (1988) Acta Histochem. 82 (1) : 5-18.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl

Neuroscience

NP_000091

P04080

Human

MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF