Recombinant humaan Cystatine C-eiwit

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. CST3 is located in the cystatin locus and is the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. Recombinant human CST3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

The IC50 value is < 2.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Cystatin C, ARMD11, MGC117328, Cystatin-C

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

The IC50 value is < 2.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

15.6 kDa (141aa) confirmed by MALDI-TOF

Cystatin C, Cystatin C, ARMD11, MGC117328, Cystatin-C, ATGP2040-10 µg, ATGP2040-20 µg, ATGP2040-50 µg, ATGP2040-100 µg, ATGP2040-250 µg, ATGP2040-500 µg, ATGP2040-1 mg, ATGP2040-10, ATGP2040-20, ATGP2040-50, ATGP2040-100, ATGP2040-250, ATGP2040-500, ATGP2040-1

Liu J. (2012) Transplant Proc. 44 (5) :1250-3.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Tags & Cell Markers

NP_000090

P01034

Human

MGSSHHHHHH SSGLVPRGSH MSSPGKPPRL VGGPMDASVE EEGVRRALDF AVGEYNKASN DMYHSRALQV VRARKQIVAG VNYFLDVELG RTTCTKTQPN LDNCPFHDQP HLKRKAFCSF QIYAVPWQGT MTLSKSTCQD A