Recombinant humaan Cystatine D-eiwit

CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population. Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

The IC50 value is < 2.0 nm. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Cystatin-D

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

The IC50 value is < 2.0 nm. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

14.36 kDa (123aa)

Cystatin D, Cystatin-D, ATGP3122-10 µg, ATGP3122-20 µg, ATGP3122-50 µg, ATGP3122-100 µg, ATGP3122-250 µg, ATGP3122-500 µg, ATGP3122-1 mg, ATGP3122-10, ATGP3122-20, ATGP3122-50, ATGP3122-100, ATGP3122-250, ATGP3122-500, ATGP3122-1

Balbin M. et al. (1994) J Biol Chem. 269:23156-23162.; ; Alvarez-Fernandez M. et al. (2005) J Biol Chem. 280:18221-18228.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Protease inhibitors

NP_001891.2

P28325

Human