Recombinant humaan Cystatine Een eiwit

CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies Recombinant human CSTA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Cystatin-A, STF1, STFA

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

13.1 kDa (118aa) confirmed by MALDI-TOF

Cystatin A, Cystatin-A, STF1, STFA, ATGP3261-10 µg, ATGP3261-20 µg, ATGP3261-50 µg, ATGP3261-100 µg, ATGP3261-250 µg, ATGP3261-500 µg, ATGP3261-1 mg, ATGP3261-10, ATGP3261-20, ATGP3261-50, ATGP3261-100, ATGP3261-250, ATGP3261-500, ATGP3261-1

Takai T., et al. (2009) J Immunol. 183 (12) :7958-65.; ; Sinha AA., et al. (2008) Anticancer Res. 28 (4B) :2271-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Cancer

NP_005204

P01040

Human

MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF