Recombinant humaan Cystatine E/M-eiwit

CST6 is a member of the type 2 cystatin family. Some of the members are active cysteine protease inhibitors, while cystatin E/M moderate the inhibition of cathepsin B but is not active against cathepsin C. CST6 is secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and the response to systemic inflammation. Recombinant human CST6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

The IC50 value is < 20 nm. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Cystatin-M, Cystatin E/M

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

The IC50 value is < 20 nm. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

15.9 kDa (142aa) confirmed by MALDI-TOF

Cystatin E, M, Cystatin-M, Cystatin E/M, ATGP1146, ATGP3263-10 µg, ATGP3263-20 µg, ATGP3263-50 µg, ATGP3263-100 µg, ATGP3263-250 µg, ATGP3263-500 µg, ATGP3263-1 mg, ATGP3263-10, ATGP3263-20, ATGP3263-50, ATGP3263-100, ATGP3263-250, ATGP3263-500, ATGP3263-1

Ni J., et al. (1997) J Biol Chem. 272 (16) :10853-8.; ; Saitoh E., et al. (1988) Biol Chem Hoppe Seyler. 369:191-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Cardiovascular

NP_001314.1

Q15828

Human

RPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM