Recombinant humaan Cystatine S-eiwit

CST4 (Cystatin-S) belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Recombinant human CST4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cystatin S

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.8 kDa (145aa) confirmed by MALDI-TOF

Cystatin S, cystatin S, ATGP1531-10 µg, ATGP1531-20 µg, ATGP1531-50 µg, ATGP1531-100 µg, ATGP1531-250 µg, ATGP1531-500 µg, ATGP1531-1 mg, ATGP1531-10, ATGP1531-20, ATGP1531-50, ATGP1531-100, ATGP1531-250, ATGP1531-500, ATGP1531-1

Saitoh E, et al. (1992) Biomed Biochim Acta 50 (4-6) : 599-605.; ; Ryan C.M., et al. (2010) J. Am. Soc. Mass Spectrom. 21:908-917

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Immunology

NP_001890

P01036

Human

MGSSHHHHHH SSGLVPRGSH MGSMSSSKEE NRIIPGGIYD ADLNDEWVQR ALHFAISEYN KATEDEYYRR PLQVLRAREQ TFGGVNYFFD VEVGRTICTK SQPNLDTCAF HEQPELQKKQ LCSFEIYEVP WEDRMSLVNS RCQEA