Recombinant humaan Cystatine S-eiwit

CST4, also known as Cystatin-S, belongs to the cystatin family. It is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. This protein is strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Recombinant human CST4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

15.4 kDa (131aa) confirmed by MALDI-TOF

Cystatin S, ATGP3223-10 µg, ATGP3223-20 µg, ATGP3223-50 µg, ATGP3223-100 µg, ATGP3223-250 µg, ATGP3223-500 µg, ATGP3223-1 mg, ATGP3223-10, ATGP3223-20, ATGP3223-50, ATGP3223-100, ATGP3223-250, ATGP3223-500, ATGP3223-1

Isemura S., et al. (1984) J. Biochem. 96:1311-1314.; ; Ryan C.M., et al. (2010) J. Am. Soc. Mass Spectrom. 21:908-917.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_001890

P01036

Human

ADPMSSSKEE NRIIPGGIYD ADLNDEWVQR ALHFAISEYN KATEDEYYRR PLQVLRAREQ TFGGVNYFFD VEVGRTICTK SQPNLDTCAF HEQPELQKKQ LCSFEIYEVP WEDRMSLVNS RCQEAHHHHH H