Recombinant humaan Cystatine SN-eiwit

CST1 (Cystatin-SN) is a member of the type 2 salivary cystatin family found in a variety of fluids and secretions, including plasma, tears, and saliva. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. CST1 was upregulated in cancerous lesions of gastric cancer tissues compared to noncancerous regions and clinicopathological analysis showed a significant correlation between high expression of CST1. Recombinant human CST1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cystatin SN, Cystain-SA-I, Cystatin-1, Cystatin-SN, Salivary cystatin-SA-1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.9 kDa (145aa) confirmed by MALDI-TOF

Cystatin SN, ATGP1332-10 µg, ATGP1332-20 µg, ATGP1332-50 µg, ATGP1332-100 µg, ATGP1332-250 µg, ATGP1332-500 µg, ATGP1332-1 mg, ATGP1332-10, ATGP1332-20, ATGP1332-50, ATGP1332-100, ATGP1332-250, ATGP1332-500, ATGP1332-1

Choi EH, et al. (2009) Clin Chim Acta. 406 (1-2) :45-51.; ; Ryan C.M., et al. (2010) J. Am. Soc. Mass Spectrom. 21:908-917

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Protease inhibitors

NP_001889

P01037

Human

WSPKEE DRIIPGGIYN ADLNDEWVQR ALHFAISEYN KATKDDYYRR PLRVLRARQQ TVGGVNYFFD VEVGRTICTK SQPNLDTCAF HEQPELQKKQ LCSFEIYEVP WENRRSLVKS RCQES