Recombinant humaan Cysteine Conjugaat beta-Lyase/CCBL1-eiwit

CCBL1 also known as Kynurenine--oxoglutrate transaminase1 isoform a. CCBL1 catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA) and it metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites. CCBL1 catalyzes the beta-elimination of S-conjugates and Se-conjugates of L- (seleno) cysteine, resulting in the cleavage of the C-S or C-Se bond. Recombinant human CCBL1 was expressed in E. coli and purified by using conventional chromatography techniques

Kynurenine-oxoglutrate transaminase1 isoform a, GTK, KAT1, KATI

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

50.3 kDa (445aa)

Cysteine Conjugate b-Lyase, CCBL1, Kynurenine-oxoglutrate transaminase1 isoform a, GTK, KAT1, KATI, ATGP3102-10 µg, ATGP3102-20 µg, ATGP3102-50 µg, ATGP3102-100 µg, ATGP3102-250 µg, ATGP3102-500 µg, ATGP3102-1 mg, ATGP3102-10, ATGP3102-20, ATGP3102-50, ATGP3102-100, ATGP3102-250, ATGP3102-500, ATGP3102-1

Han Q., et al. (2009) J. Med. Chem. 52:2786-2793.; ; Rossi F., et al. (2004) J. Biol. Chem. 279:50214-50220.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Metabolism

NP_004050

Q16773

Human

MAKQLQA RRLDGIDYNP WVEFVKLASE HDVVNLGQGF PDFPPPDFAV EAFQHAVSGD FMLNQYTKTF GYPPLTKILA SFFGELLGQE IDPLRNVLVT VGGYGALFTA FQALVDEGDE VIIIEPFFDC YEPMTMMAGG RPVFVSLKPG PIQNGELGSS SNWQLDPMEL AGKFTSRTKA LVLNTPNNPL GKVFSREELE LVASLCQQHD VVCITDEVYQ WMVYDGHQHI SIASLPGMWE RTLTIGSAGK TFSATGWKVG WVLGPDHIMK HLRTVHQNSV FHCPTQSQAA VAESFEREQL LFRQPSSYFV QFPQAMQRCR DHMIRSLQSV GLKPIIPQGS YFLITDISDF KRKMPDLPGA VDEPYDRRFV KWMIKNKGLV AIPVSIFYSV PHQKHFDHYI RFCFVKDEAT LQAMDEKLRK WKVEL