Recombinant humaan CYTH3-eiwit

CYTH3 is a member of PSCD family. Members of PSCD family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain, and appear to mediate the regulation of protein sorting and membrane trafficking. CYTH3 is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1. Recombinant human CYTH3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytohesin-3, Cytohesin 3, ARNO3, GRP1, PSCD3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BRADFORD assay)

> 90% by SDS-PAGE

48.7 kDa (422aa) confirmed by MALDI-TOF

CYTH3, Cytohesin-3, Cytohesin 3, ARNO3, GRP1, PSCD3, ATGP2658-10 µg, ATGP2658-20 µg, ATGP2658-50 µg, ATGP2658-100 µg, ATGP2658-250 µg, ATGP2658-500 µg, ATGP2658-1 mg, ATGP2658-10, ATGP2658-20, ATGP2658-50, ATGP2658-100, ATGP2658-250, ATGP2658-500, ATGP2658-1

Klarlund JK, Guilherme A, et al. (1997) Science 275 (5308) : 1927-30.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_004218

O43739

Human

MGSSHHHHHH SSGLVPRGSH MGSMDEDGGG EGGGVPEDLS LEEREELLDI RRRKKELIDD IERLKYEIAE VMTEIDNLTS VEESKTTQRN KQIAMGRKKF NMDPKKGIQF LIENDLLQSS PEDVAQFLYK GEGLNKTVIG DYLGERDEFN IKVLQAFVEL HEFADLNLVQ ALRQFLWSFR LPGEAQKIDR MMEAFASRYC LCNPGVFQST DTCYVLSFAI IMLNTSLHNH NVRDKPTAER FIAMNRGINE GGDLPEELLR NLYESIKNEP FKIPEDDGND LTHTFFNPDR EGWLLKLGGR VKTWKRRWFI LTDNCLYYFE YTTDKEPRGI IPLENLSIRE VEDPRKPNCF ELYNPSHKGQ VIKACKTEAD GRVVEGNHVV YRISAPSPEE KEEWMKSIKA SISRDPFYDM LATRKRRIAN KK