Recombinant humaan cytochroom p450 reductase/POR-eiwit

POR, also known as NADPH--cytochrome P450 reductase, is a flavoprotein that donates electrons to all microsomal P450 enzymes. POR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. It is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase and flavodoxin. Recombinant human POR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Cytochrome p450 oxidoreductase, NADPH--cytochrome P450 reductase, NADPH--hemoprotein reductase, CYPOR, CPR, P450R

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

77.9 kDa (686aa)

POR, Cytochrome p450 oxidoreductase, NADPH--cytochrome P450 reductase, NADPH--hemoprotein reductase, CYPOR, CPR, P450R, NADPH-cytochrome P450 reductase, NADPH-hemoprotein reductase, NADPH cytochrome P450 reductase, NADPH hemoprotein reductase, ATGP3921-10 µg, ATGP3921-20 µg, ATGP3921-50 µg, ATGP3921-100 µg, ATGP3921-250 µg, ATGP3921-500 µg, ATGP3921-1 mg, ATGP3921-010, ATGP3921-020, ATGP3921-050, ATGP3921-100, ATGP3921-250, ATGP3921-500, ATGP3921-01M

Shen A L, et al. (1989) . J Biol Chem. 264:7584-7589.; ; Haniju M, et al. (1989) . Biochemistry. 28:8639-8645.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_000932

P16435

Human

Met1