Recombinant humaan cytoglobine/CYGB-eiwit

CYGB is a globin molecule located in the brain and most notably utilized in marine mammals. It is thought to be a method of protection under conditions of hypoxia. The predicted function of cytoglobin is the modulation of oxygen and nitric oxide metabolism or scavenging free radicals within a cell. Recombinant human CYGB, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

CYGB, Cytoglobin, HGB, Histoglobin, STAP, Stellate cell activation associated protein

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.5 kDa (210aa) confirmed by MALDI-TOF

CYGB, Cytoglobin, HGB, STAP, Cytoglobin, CYGB, Histoglobin, Stellate cell activation associated protein., ATGP0592-10 µg, ATGP0592-20 µg, ATGP0592-50 µg, ATGP0592-100 µg, ATGP0592-250 µg, ATGP0592-500 µg, ATGP0592-1 mg, ATGP0592-10, ATGP0592-20, ATGP0592-50, ATGP0592-100, ATGP0592-250, ATGP0592-500, ATGP0592-1

Hankeln T., et al. (2002) Mol Biol Evol. 19 (4) :416-21; ; Gazdar AF., et al. (2008) Cancer Res. 68 (18) :7448-56;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Redox Proteins

AAH29798

Q8WWM9

Human

MEKVPGEMEI ERRERSEELS EAERKAVQAM WARLYASCED VGVAILVRFF VNFPSAKQYF SQFKHMEDPL EMERSPQLRK HACRVMGALN TVVENLHDPD KVSSVLALVG KAHALKHKVE PVYFKILSGV ILEVVAEEFA SDFPPETQRA WAKLRGLIYS HVTAAYKEVG WVQQVPNATT PPATLPSSGP