Recombinant humaan cytokeratine 20/KRT20-eiwit

KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. KRT20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. Recombinant human KRT20 protein, fused to His-tag at N-terminus, was expressed in E. coli.

CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

50.9 kDa (447aa)

KRT20, Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20; CK-20; CK20; K20; KRT21, ATGP1960-10 µg, ATGP1960-20 µg, ATGP1960-50 µg, ATGP1960-100 µg, ATGP1960-250 µg, ATGP1960-500 µg, ATGP1960-1 mg, ATGP1960-10, ATGP1960-20, ATGP1960-50, ATGP1960-100, ATGP1960-250, ATGP1960-500, ATGP1960-1

Schweizer J, Bowden PE, et al. (2006) . J Cell Biol.174 (2) :169-74.

Hoshino A, et al. Identification of autoantibodies using human proteome microarrays in patients with IPEX syndrome. (Clin Immunol. 2019) {https://pubmed.ncbi.nlm.nih.gov/30951839/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Cytoskeleton

NP_061883

P35900

Human

MDFSRRS FHRSLSSSLQ APVVSTVGMQ RLGTTPSVYG GAGGRGIRIS NSRHTVNYGS DLTGGGDLFV GNEKMAMQNL NDRLASYLEK VRTLEQSNSK LEVQIKQWYE TNAPRAGRDY SAYYRQIEEL RSQIKDAQLQ NARCVLQIDN AKLAAEDFRL KYETERGIRL TVEADLQGLN KVFDDLTLHK TDLEIQIEEL NKDLALLKKE HQEEVDGLHK HLGNTVNVEV DAAPGLNLGV IMNEMRQKYE VMAQKNLQEA KEQFERQTAV LQQQVTVNTE ELKGTEVQLT ELRRTSQSLE IELQSHLSMK ESLEHTLEET KARYSSQLAN LQSLLSSLEA QLMQIRSNME RQNNEYHILL DIKTRLEQEI ATYRRLLEGE DVKTTEYQLS TLEERDIKKT RKIKTVVQEV VDGKVVSSEV KEVEENI