Recombinant humaan cytosolisch bèta-Glucosidase/GBA3-eiwit

GBA3 is an enzyme that can hydrolyze several types of glycosides. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Recombinant human GBA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytosolic beta-glucosidase, CBG, CBGL1, GLuC, KLRP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

56.1 kDa (492aa)

Cytosolic b-Glucosidase, GBA3, Cytosolic beta-glucosidase, CBG, CBGL1, GLuC, KLRP, ATGP2849-10 µg, ATGP2849-20 µg, ATGP2849-50 µg, ATGP2849-100 µg, ATGP2849-250 µg, ATGP2849-500 µg, ATGP2849-1 mg, ATGP2849-10, ATGP2849-20, ATGP2849-50, ATGP2849-100, ATGP2849-250, ATGP2849-500, ATGP2849-1

Berrin J.-G., et al, (2002) Eur. J. Biochem. 269:249-258.; ; Nemeth K., et al, (2003) Eur. J. Nutr. 42:29-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Metabolism

NP_066024

Q9H227

Human

MAFPAGF GWAAATAAYQ VEGGWDADGK GPCVWDTFTH QGGERVFKNQ TGDVACGSYT LWEEDLKCIK QLGLTHYRFS LSWSRLLPDG TTGFINQKGI DYYNKIIDDL LKNGVTPIVT LYHFDLPQTL EDQGGWLSEA IIESFDKYAQ FCFSTFGDRV KQWITINEAN VLSVMSYDLG MFPPGIPHFG TGGYQAAHNL IKAHARSWHS YDSLFRKKQK GMVSLSLFAV WLEPADPNSV SDQEAAKRAI TFHLDLFAKP IFIDGDYPEV VKSQIASMSQ KQGYPSSRLP EFTEEEKKMI KGTADFFAVQ YYTTRLIKYQ ENKKGELGIL QDAEIEFFPD PSWKNVDWIY VVPWGVCKLL KYIKDTYNNP VIYITENGFP QSDPAPLDDT QRWEYFRQTF QELFKAIQLD KVNLQVYCAW SLLDNFEWNQ GYSSRFGLFH VDFEDPARPR VPYTSAKEYA KIIRNNGLEA HL