Recombinant humaan D aminozuuroxidase/DAO-eiwit

D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 3.5 units/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.

D-amino acid oxidase, DAMOX, DAO, OXDA, DAAO

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 3.5unit/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.

41.6 kDa (367aa) confirmed by MALDI-TOF

DAAO, D-amino acid oxidase, DAMOX, DAO, OXDA, ATGP3237-10 µg, ATGP3237-20 µg, ATGP3237-50 µg, ATGP3237-100 µg, ATGP3237-250 µg, ATGP3237-500 µg, ATGP3237-1 mg, ATGP3237-10, ATGP3237-20, ATGP3237-50, ATGP3237-100, ATGP3237-250, ATGP3237-500, ATGP3237-1

Kawazoe T., et al. (2006) . Protein Sci. 15 (12) :2708-17.; ; Chassande O., et al. (1994) . J Biol Chem. 269 (20) :14484-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT

Redox Proteins

NP_001908

P14920

Human

MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL