Recombinant humaan DAN-eiwit

Neuroblastoma suppressor of tumorigenicity 1, also known as NBL1, is a member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. NBL1 is a candidate as a tumor suppressor of neuroblastoma and may play a role in preventing cells from entering the final stage (G1/S) of the transformation process. It is produced in small neurons of the dorsal root ganglion and its expression is activated by MATH-1. Recombinant human NBL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neuroblastoma suppressor of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

20 kDa (187aa) confirmed by MALDI-TOF

DAN, Neuroblastoma suppressor of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3, ATGP1967-10 µg, ATGP1967-20 µg, ATGP1967-50 µg, ATGP1967-100 µg, ATGP1967-250 µg, ATGP1967-500 µg, ATGP1967-1 mg, ATGP1967-10, ATGP1967-20, ATGP1967-50, ATGP1967-100, ATGP1967-250, ATGP1967-500, ATGP1967-1

Kim A S., et al. (2003) Brain Res Dev. 145:159-162; ; Gerlach Bank L M., et al. (2004) Dev Dyn. 229: 219-230.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Cancer

NP_005371

P41271

Human

APPPINK LALFPDKSAW CEAKNITQIV GHSGCEAKSI QNRACLGQCF SYSVPNTFPQ STESLVHCDS CMPAQSMWEI VTLECPGHEE VPRVDKLVEK ILHCSCQACG KEPSHEGLSV YVQGEDGPGS QPGTHPHPHP HPHPGGQTPE PEDPPGAPHT EEEGAED