Recombinant humaan DCAF7-eiwit

DCAF7, also known as DDB1-and CuL4-associated factor, involved in craniofacial development. This protein acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. This protein associates with DIAPH1 and controls GLI1 transcriptional activity. This protein could be involved in normal and disease skin development. Recombinant human DCAF7 protein, fused to His-tag at N-terminus, was expressed in E. coli and denatured using detergent during conventional chromatography purification process.

DDB1-and CuL4-associated factor 7, AN11, HAN11, SWAN-1, WDR68

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.6 kDa (298aa)

DCAF7, DDB1-and CuL4-associated factor 7, AN11, HAN11, SWAN-1, WDR68, ATGP1177-10 µg, ATGP1177-20 µg, ATGP1177-50 µg, ATGP1177-100 µg, ATGP1177-250 µg, ATGP1177-500 µg, ATGP1177-1 mg, ATGP1177-10, ATGP1177-20, ATGP1177-50, ATGP1177-100, ATGP1177-250, ATGP1177-500, ATGP1177-1

Morita K., et al. (2006) J. Dermatol. Sci. 44:11-20; ; Jin J., et al. (2006) Mol. Cell. 23:709-721

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 1mM DTT

Development

NP_005819

P61962

Human

MGSSHHHHHH SSGLVPRGSH MVYAMNWSVR PDKRFRLALG SFVEEYNNKV QLVGLDEESS EFICRNTFDH PYPTTKLMWI PDTKGVYPDL LATSGDYLRV WRVGETETRL ECLLNNNKNS DFCAPLTSFD WNEVDPYLLG TSSIDTTCTI WGLETGQVLG RVNLVSGHVK TQLIAHDKEV YDIAFSRAGG GRDMFASVGA DGSVRMFDLR HLEHSTIIYE DPQHHPLLRL CWNKQDPNYL ATMAMDGMEV VILDVRVPCT PVARLNNHRA CVNGIAWAPH SSCHICTAAD DHQALIWD