Recombinant humaan DCI/ECI1-eiwit

ECI1 is a member of the hydratase/isomerase superfamily. The protein is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. Recombinant human ECI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Enoyl-CoA delta isomerase 1, DCI, 3,2-trans-enoyl-CoA isomerase, Delta (3), Delta (2) -enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.1 kDa (284aa) confirmed by MALDI-TOF

ECI1, enoyl-CoA delta isomerase 1, DCI, ATGP2445-10 µg, ATGP2445-20 µg, ATGP2445-50 µg, ATGP2445-100 µg, ATGP2445-250 µg, ATGP2445-500 µg, ATGP2445-1 mg, ATGP2445-10, ATGP2445-20, ATGP2445-50, ATGP2445-100, ATGP2445-250, ATGP2445-500, ATGP2445-1

Janssen u., et al (1994) . Genomics 23:223-228; ; Takahashi Y., et al (1994) . Biochem. J. 304:849-852

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_001910

P42126

Human

MGSSHHHHHH SSGLVPRGSH MGSFGSQRVL VEPDAGAGVA VMKFKNPPVN SLSLEFLTEL VISLEKLEND KSFRGVILTS DRPGVFSAGL DLTEMCGRSP AHYAGYWKAV QELWLRLYQS NLVLVSAING ACPAGGCLVA LTCDYRILAD NPRYCIGLNE TQLGIIAPFW LKDTLENTIG HRAAERALQL GLLFPPAEAL QVGIVDQVVP EEQVQSTALS AIAQWMAIPD HARQLTKAMM RKATASRLVT QRDADVQNFV SFISKDSIQK SLQMYLERLK EEKG