Recombinant humaan DCK-eiwit

DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or uTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Deoxycytidine kinase, MGC117410, MGC138632, Deoxycytidine kinase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.6 kDa (296aa) confirmed by MALDI-TOF

DCK, ATGP0738-10 µg, ATGP0738-20 µg, ATGP0738-50 µg, ATGP0738-100 µg, ATGP0738-250 µg, ATGP0738-500 µg, ATGP0738-1 mg, ATGP0738-10, ATGP0738-20, ATGP0738-50, ATGP0738-100, ATGP0738-250, ATGP0738-500, ATGP0738-1

Radu CG., et al. (2010) Proc Natl Acad Sci u S A. 107 (12) :5551-6; ; Ribeiro R., et al. (2007) J Pharmacol Exp Ther. 323 (3) :935-45;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, 10% glycerol

Enzymes & Proteases

NP_000779.1

P27707

Human

MATP PKRSCPSFSA SSEGTRIKKI SIEGNIAAGK STFVNILKQL CEDWEVVPEP VARWCNVQST QDEFEELTMS QKNGGNVLQM MYEKPERWSF TFQTYACLSR IRAQLASLNG KLKDAEKPVL FFERSVYSDR YIFASNLYES ECMNETEWTI YQDWHDWMNN QFGQSLELDG IIYLQATPET CLHRIYLRGR NEEQGIPLEY LEKLHYKHES WLLHRTLKTN FDYLQEVPIL TLDVNEDFKD KYESLVEKVK EFLSTL