Recombinant humaan DCNL2/DCUN1D2-eiwit

DCN1-like protein 2, also known as DCuN1D2, which contains one DCuN1 domain and 1 uBA-like domain, may play a role in the neddylation of cullins that regulate SCF-type ubiquitin ligase complexes. The gene encoding Dcun1D2 exists on human chromosome 13. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Recombinant human DCuN1D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Defective in cullin neddylation 1 domain containing 2, DCUN1 domain-containing protein 2, DCN1-like protein 2, C13orf17, DCUN1L2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32.3 kDa (279aa) confirmed by MALDI-TOF

DCuN1D2, defective in cullin neddylation protein 1 (DCN1) -like protein 2, DCuN1L2, ATGP1158-10 µg, ATGP1158-20 µg, ATGP1158-50 µg, ATGP1158-100 µg, ATGP1158-250 µg, ATGP1158-500 µg, ATGP1158-1 mg, ATGP1158-10, ATGP1158-20, ATGP1158-50, ATGP1158-100, ATGP1158-250, ATGP1158-500, ATGP1158-1

Hassler M., et al. (2007) Mol Cell. 28:371-385.; ; Dunham A., et al. (2004) Nature. 428:522-528.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Ubiquitination

NP_001014305

Q6PH85

Human

MGSSHHHHHH SSGLVPRGSH MHKLKSSQKD KVRQFMACTQ AGERTAIYCL TQNEWRLDEA TDSFFQNPDS LHRESMRNAV DKKKLERLYG RYKDPQDENK IGVDGIQQFC DDLSLDPASI SVLVIAWKFR AATQCEFSRK EFLDGMTELG CDSMEKLKAL LPRLEQELKD TAKFKDFYQF TFTFAKNPGQ KGLDLEMAVA YWKLVLSGRF KFLDLWNTFL MEHHKRSIPR DTWNLLLDFG NMIADDMSNY DEEGAWPVLI DDFVEYARPV VTGGKRSLF