Recombinant humaan DCPS-eiwit

DCPS, also known as scavenger mRNA-decapping enzyme, is belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3'-5' degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain. Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Scavenger mRNA-decapping enzyme DcpS, DCS1, HINT5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

40.7 kDa (357aa) confirmed by MALDI-TOF

DCPS, Scavenger mRNA-decapping enzyme DcpS, DCS1, HINT5, ATGP1162-10 µg, ATGP1162-20 µg, ATGP1162-50 µg, ATGP1162-100 µg, ATGP1162-250 µg, ATGP1162-500 µg, ATGP1162-1 mg, ATGP1162-10, ATGP1162-20, ATGP1162-50, ATGP1162-100, ATGP1162-250, ATGP1162-500, ATGP1162-1

Liu H., et al. (2002) EMBO J. 21:4699-4708; ; Kwasnicka D.A., et al. (2003) J. Biol. Chem. 278:39051-39058

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_054745

Q96C86

Human

MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPDPSDGF VLIPDLKWNQ QQLDDLYLIA ICHRRGIRSL RDLTPEHLPL LRNILHQGQE AILQRYRMKG DHLRVYLHYL PSYYHLHVHF TALGFEAPGS GVERAHLLAE VIENLECDPR HYQQRTLTFA LRADDPLLKL LQEAQQS