Recombinant humaan DCT-eiwit

DCT, as known as L-dopachrome tautomerase isoform 1, is the tyrosinase family members. This protein plays a key role in the synthesis of the melanin pigment including tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1) . These are strongly implicated in eumelanin synthesis. The switch between the eumelanin and the pheomelanin pathways is thought to depend on the presence of cysteine. Thus, in the absence of cysteine, dopaquinone, the product of tyrosinase action, is transformed to cyclodopa (leucodopachrome) and then to dopachrome (and DOPA) . Recombinant human DCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

L-dopachrome tautomerase isoform 1, DCT, TRP-2, TYRP2

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

52.1 kDa (455aa)

DCT, L-dopachrome tautomerase isoform 1 , DCT, TRP-2, TYRP2, ATGP3454-10 µg, ATGP3454-20 µg, ATGP3454-50 µg, ATGP3454-100 µg, ATGP3454-250 µg, ATGP3454-500 µg, ATGP3454-1 mg, ATGP3454-10, ATGP3454-20, ATGP3454-50, ATGP3454-100, ATGP3454-250, ATGP3454-500, ATGP3454-1

Ainger SA., et al, (2014) Exp. Dermatol. 23:916-921.; ; Guyonneau L., et al, (2004) Mol. Cell. Biol. 8:3396-3403.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_001913

P40126

Human

Gln24