Recombinant humaan DCTPP1-eiwit

DCTPP1, also known as dCTP pyrophosphatase 1, contains an epilepsy-associated repeat (EAR) domain that is predicted to participate in protein-protein interactions, most likely involved in ligand recognition. This protein functions to hydrolyze abnormal nucleotide triphosphates (NTPs) in cancer cells that, if unregulated, could be incorporated into nascent DNA or RNA. Recombinant human DCTPP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Deoxycytidine-triphosphatase 1, dCTPase 1, dCTP pyrophosphatase 1, CDA03, RS21C6, XTP3TPA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.2 kDa (194aa) confirmed by MALDI-TOF

DCTPP1, dCTP pyrophosphatase 1, CDA03, RS21C6, XTP3TPA, ATGP1381-10 µg, ATGP1381-20 µg, ATGP1381-50 µg, ATGP1381-100 µg, ATGP1381-250 µg, ATGP1381-500 µg, ATGP1381-1 mg, ATGP1381-10, ATGP1381-20, ATGP1381-50, ATGP1381-100, ATGP1381-250, ATGP1381-500, ATGP1381-1

Moroz O.V. et al. (2005) J. Mol. Biol. 347: 243-255.; ; Rual J.F. et al. (2005) Nature 437:1173-1178.

Kawamura T, et al. Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival. (Sci Rep. 2016) {https://pubmed.ncbi.nlm.nih.gov/27210421/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_077001

Q9H773

Human

MSVAGG EIRGDTGGED TAAPGRFSFS PEPTLEDIRR LHAEFAAERD WEQFHQPRNL LLALVGEVGE LAELFQWKTD GEPGPQGWSP RERAALQEEL SDVLIYLVAL AARCRVDLPL AVLSKMDINR RRYPAHLARS SSRKYTELPH GAISEDQAVG PADIPCDSTG QTST