Recombinant humaan DCXR-eiwit

Dicarbonyl/L-xylulose reductase, also known as DCXR, is an enzyme responsible for the metabolism of xylulose, converting it into xylitol. DCXR was expressed at low levels and was localized predominantly in the cytoplasmic membrane. In contrast, in virtually all grades of early-stage prostate cancer and in all chemohormonally treated cases, DCXR was strikingly overexpressed and was localized predominantly in the cytoplasm and nucleus. Recombinant human DCXR, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 1,800 pmol/min/ug and is defined as the amount of enzyme that oxidize 1pmole of xylitol to L-xylulose per minute at pH 10.0 at 37C.

Carbonyl reductase II, DCR, Dicarbonyl/L-xylulose reductase, HCR 2, HCR II, KIDCR, Kidney dicarbonyl reductase, L-xylulose reductase, P34H, Sperm surface protein P34H, XR

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 1,800pmol/min/ug and is defined as the amount of enzyme that oxidize 1pmole of xylitol to L-xylulose per minute at pH 10.0 at 37C.

28 kDa (264aa) confirmed by MALDI-TOF

Carbonyl reductase II, DCR, DCXR, Dicarbonyl/L-xylulose reductase, HCR 2, HCR II, HCR2, HCR-2, HCRII, HCR-II, KIDCR, Kidney dicarbonyl reductase, L xylulose reductase, L-xylulose reductase, P34H, Sperm surface protein P34H, XR, ATGP3527-10 µg, ATGP3527-20 µg, ATGP3527-50 µg, ATGP3527-100 µg, ATGP3527-250 µg, ATGP3527-500 µg, ATGP3527-1 mg, ATGP3527-010, ATGP3527-020, ATGP3527-050, ATGP3527-100, ATGP3527-250, ATGP3527-500, ATGP3527-01M

Cho Veqa JH., et al. (2007) Cancer Epidemiol Biomakers Prev. 16 (12) :2615-22.; ; Nakaqawa J., et al. (2002) J Biol Chem. 277 (20) :17883-91.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl

Metabolism

NP_057370

Q7Z4W1

Human

MGSSHHHHHH SSGLVPRGSH MELFLAGRRV LVTGAGKGIG RGTVQALHAT GARVVAVSRT QADLDSLVRE CPGIEPVCVD LGDWEATERA LGSVGPVDLL VNNAAVALLQ PFLEVTKEAF DRSFEVNLRA VIQVSQIVAR GLIARGVPGA IVNVSSQCSQ RAVTNHSVYC STKGALDMLT KVMALELGPH KIRVNAVNPT VVMTSMGQAT WSDPHKAKTM LNRIPLGKFA EVEHVVNAIL FLLSDRSGMT TGSTLPVEGG FWAC