Recombinant humaan DDAH1-eiwit

Dimethylarginine dimethylaminohydrolase 1, also known DDAH1, belongs to the dimethylarginine dimethylaminohydrolase gene family. The DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Impairment of DDAH1 causes ADMA (asymmetric dimethylarginine) accumulation and a reduction in cGMP generation. Recombinant human DDAH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, Dimethylargininase-1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.5 kDa (308aa) confirmed by MALDI-TOF

DDAH1, Dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, Dimethylargininase-1, ATGP0754-10 µg, ATGP0754-20 µg, ATGP0754-50 µg, ATGP0754-100 µg, ATGP0754-250 µg, ATGP0754-500 µg, ATGP0754-1 mg, ATGP0754-10, ATGP0754-20, ATGP0754-50, ATGP0754-100, ATGP0754-250, ATGP0754-500, ATGP0754-1

NaKagomi S., et al. (1999) Eur J Neurosci. 11:2160-2166.; ; Leiper J., et al. (2002) J Biol Chem. 276:40449-40456.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_036269.1

O94760

Human

MAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS