Recombinant humaan DDIT4-eiwit

DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA damage-inducible transcript 4 protein, Dig2, FLJ20500, REDD1, RP11-442H21.1, RTP801

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

DDIT4, DNA damage-inducible transcript 4 protein, Dig2, FLJ20500, REDD1, RP11-442H21.1, RTP801, ATGP0896-10 µg, ATGP0896-20 µg, ATGP0896-50 µg, ATGP0896-100 µg, ATGP0896-250 µg, ATGP0896-500 µg, ATGP0896-1 mg, ATGP0896-10, ATGP0896-20, ATGP0896-50, ATGP0896-100, ATGP0896-250, ATGP0896-500, ATGP0896-1

Yoshida T., et al. (2010) Nat Med. 16 (7) :767-73.; ; Regazzetti C., et al. (2010) J Biol Chem. 285 (8) :5157-64.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Cancer

NP_061931

Q9NX09

Human

MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC