Recombinant humaan DDT-eiwit

DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5, 6-dihydroxyindole (DHI) . This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

D-dopachrome tautomerase, DDCT, D-dopachtome decarboxylase, D-dopachrome tautomerase, D dopachrome decarboxylase, D dopachrome tautomerase, Phenylpyruvate tautomerase II

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

14.8 kDa (138aa) confirmed by MALDI-TOF

DDT, D-dopachrome tautomerase, DDCT, D-dopachtome decarboxylase, D-dopachrome tautomerase, D dopachrome decarboxylase, D dopachrome tautomerase, Phenylpyruvate tautomerase II, ATGP0593-10 µg, ATGP0593-20 µg, ATGP0593-50 µg, ATGP0593-100 µg, ATGP0593-250 µg, ATGP0593-500 µg, ATGP0593-1 mg, ATGP0593-10, ATGP0593-20, ATGP0593-50, ATGP0593-100, ATGP0593-250, ATGP0593-500, ATGP0593-1

Arisawa K., et al. (2009) Toxicology. 255 (1-2) :6-14.; ; Nishihira J., et al. (1999) Biochemistry. 38 (11) :3268-79.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_001346

P30046

Human

MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL