Recombinant humaan DDX56-eiwit

DDX56 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX56 shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. Recombinant human DDX56 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Probable ATP-dependent RNA helicase DDX56 isoform 1, DDX21, DDX26, NOH61

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

64.0 kDa (570aa)

DDX56, probable ATP-dependent RNA helicase DDX56 isoform 1, DDX21, DDX26, NOH61, ATGP2358-10 µg, ATGP2358-20 µg, ATGP2358-50 µg, ATGP2358-100 µg, ATGP2358-250 µg, ATGP2358-500 µg, ATGP2358-1 mg, ATGP2358-10, ATGP2358-20, ATGP2358-50, ATGP2358-100, ATGP2358-250, ATGP2358-500, ATGP2358-1

Matsuoka S., et al (2007) . Science 316:1160-1166

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Epigenomics (Transcription & Translation)

NP_061955

Q9NY93

Human

MGSSHHHHHH SSGLVPRGSH MGSMEDSEAL GFEHMGLDPR LLQAVTDLGW SRPTLIQEKA IPLALEGKDL LARARTGSGK TAAYAIPMLQ LLLHRKATGP VVEQAVRGLV LVPTKELARQ AQSMIQQLAT YCARDVRVAN VSAAEDSVSQ RAVLMEKPDV VVGTPSRILS HLQQDSLKLR DSLELLVVDE ADLLFSFGFE EELKSLLCHL PRIYQAFLMS ATFNEDVQAL KELILHNPVT LKLQESQLPG PDQLQQFQVV CETEEDKFLL LYALLKLSLI RGKSLLFVNT LERSYRLRLF LEQFSIPTCV LNGELPLRSR CHIISQFNQG FYDCVIATDA EVLGAPVKGK RRGRGPKGDK ASDPEAGVAR GIDFHHVSAV LNFDLPPTPE AYIHRAGRTA RANNPGIVLT FVLPTEQFHL GKIEELLSGE NRGPILLPYQ FRMEEIEGFR YRCRDAMRSV TKQAIREARL KEIKEELLHS EKLKTYFEDN PRDLQLLRHD LPLHPAVVKP HLGHVPDYLV PPALRGLVRP HKKRKKLSSS CRKAKRAKSQ NPLRSFKHKG KKFRPTAKPS