Recombinant humaan DECR1-eiwit

DECR1 is a mitochondrial protein that exists as a homotetramer and belongs to the family of short-chain dehydrogenases/reductases. It functions as an auxiliary enzyme of beta-oxidation andt participates in the metabolism of unsaturated fatty enoyl-CoA esters. Specifically, DECR1 uses NADP+ to catalyze the reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA, which can then be used as an intermediate in the Krebs cycle. Additionally, DECR1 is thought to function as a tumor suppressor, possibly downregulating the expression of Neu and slowing the rate of tumorigenesis. Recombinant human DECR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

24-dienoyl-CoA reductase mitochondrial, 2,4-dienoyl-CoA reductase, mitochondrial, DECR, NADPH, SDR18C1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.4 kDa (322aa) confirmed by MALDI-TOF

DECR1, 24-dienoyl-CoA reductase mitochondrial, 2,4-dienoyl-CoA reductase, mitochondrial, DECR, NADPH, SDR18C1, ATGP0984-10 µg, ATGP0984-20 µg, ATGP0984-50 µg, ATGP0984-100 µg, ATGP0984-250 µg, ATGP0984-500 µg, ATGP0984-1 mg, ATGP0984-10, ATGP0984-20, ATGP0984-50, ATGP0984-100, ATGP0984-250, ATGP0984-500, ATGP0984-1

Koivuranta KT., et al. (1994) Biochem J. 304 (3) :787-92. ; ; Roe CR., et al. (1990) J Clin Invest. 85 (5) :1703-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Cancer

NP_001350

Q16698

Human

NTEALQSKF FSPLQKAMLP PNSFQGKVAF ITGGGTGLGK GMTTLLSSLG AQCVIASRKM DVLKATAEQI SSQTGNKVHA IQCDVRDPDM VQNTVSELIK VAGHPNIVIN NAAGNFISPT ERLSPNAWKT ITDIVLNGTA FVTLEIGKQL IKAQKGAAFL SITTIYAETG SGFVVPSASA KAGVEAMSKS LAAEWGKYGM RFNVIQPGPI KTKGAFSRLD PTGTFEKEMI GRIPCGRLGT VEELANLAAF LCSDYASWIN GAVIKFDGGE EVLISGEFND LRKVTKEQWD TIEELIRKTK GS