Recombinant humaan DECR2-eiwit

DECR2, also known as peroxisomal 2, 4-dienoyl-CoA reductase, is an auxiliary enzyme of beta-oxidation. DECR2 participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. This protein catalyzes the NADP-dependent reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA. Recombinant human DECR2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Peroxisomal 24-dienoyl-CoA reductase, Peroxisomal 2,4-dienoyl-CoA reductase, PDCR, SDR17C1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.2 kDa (315aa) confirmed by MALDI-TOF

DECR2, Peroxisomal 24-dienoyl-CoA reductase, Peroxisomal 2,4-dienoyl-CoA reductase, PDCR, SDR17C1, ATGP1330-10 µg, ATGP1330-20 µg, ATGP1330-50 µg, ATGP1330-100 µg, ATGP1330-250 µg, ATGP1330-500 µg, ATGP1330-1 mg, ATGP1330-10, ATGP1330-20, ATGP1330-50, ATGP1330-100, ATGP1330-250, ATGP1330-500, ATGP1330-1

De Nys K., et al. (2001) Biochim. Biophys. Acta. 1533:66-72; ; Daniels R.J., et al. (2001) Hum. Mol. Genet. 10:339-352

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Metabolism

NP_065715

Q9NUI1

Human

MAQPPPD VEGDDCLPAY RHLFCPDLLR DKVAFITGGG SGIGFRIAEI FMRHGCHTVI ASRSLPRVLT AARKLAGATG RRCLPLSMDV RAPPAVMAAV DQALKEFGRI DILINCAAGN FLCPAGALSF NAFKTVMDID TSGTFNVSRV LYEKFFRDHG GVIVNITATL GNRGQALQVH AGSAKAAVDA MTRHLAVEWG PQNIRVNSLA PGPISGTEGL RRLGGPQASL STKVTASPLQ RLGNKTEIAH SVLYLASPLA SYVTGAVLVA DGGAWLTFPN GVKGLPDFAS FSAKL