Recombinant humaan DEDD-eiwit

Death effector domain-containing protein, also known as DEDD, is a cytoplasmic protein. It translocates to the nucleus during CD95-mediated apoptosis, where it localizes to nucleoli-like structures, activates caspase-6 and specifically inhibits RNA polymerase I-dependent transcription. The cell death activity of DEDD relates to its nuclear localization. DEDD is widely expressed in a variety of tissues, with highest levels in the testis. Overexpression of this gene was shown to induce weak apoptosis. Recombinant human DEDD protein, fused to His-tag at N-terminus, was expressed in E. coli.

Death effector domain-containing protein, CASP8IP1, DEDD1, DEFT, FLDED1, KE05

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

38.9 kDa (338aa)

DEDD, Death effector domain-containing protein, CASP8IP1, DEDD1, DEFT, FLDED1, KE05, ATGP1945-10 µg, ATGP1945-20 µg, ATGP1945-50 µg, ATGP1945-100 µg, ATGP1945-250 µg, ATGP1945-500 µg, ATGP1945-1 mg, ATGP1945-10, ATGP1945-20, ATGP1945-50, ATGP1945-100, ATGP1945-250, ATGP1945-500, ATGP1945-1

Stegh A H., et al. (1998) EMBO J. 17:5974-5986.; ; Schickling O., et al. (2001) Cell Death Differ. 8:1157-1168.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Apoptosis

NP_127491

O75618

Human

MAGLKRRASQ VWPEEHGEQE HGLYSLHRMF DIVGTHLTHR DVRVLSFLFV DVIDDHERGL IRNGRDFLLA LERQGRCDES NFRQVLQLLR IITRHDLLPY VTLKRRRAVC PDLVDKYLEE TSIRYVTPRA LSDPEPRPPQ PSKTVPPHYP VVCCPTSGPQ MCSKRPARGR ATLGSQRKRR KSVTPDPKEK QTCDIRLRVR AEYCQHETAL QGNVFSNKQD PLERQFERFN QANTILKSRD LGSIICDIKF SELTYLDAFW RDYINGSLLE ALKGVFITDS LKQAVGHEAI KLLVNVDEED YELGRQKLLR NLMLQALP